Journal: Journal of Clinical Microbiology

Article Title: Real-Time Optical Antimicrobial Susceptibility Testing

doi: 10.1128/JCM.00440-13

Figure Lengend Snippet: The oCelloScope detection system. (a) Picture of the detection system with a standard 96-well plate inserted. (b) Simplified engineering drawing of the detection principle. A volume of 50 μl of a growing bacterial culture is scanned, resulting in a stack of images. Each image can be transformed to a two-dimensional (2D) picture. (c) 2D picture of S. alactolyticus from the scanning process. The magnitude is comparable to an ×200 magnification in a standard light microscope.

Article Snippet: The common Gram-positive pig gut bacterium Streptococcus alactolyticus (type strain DSM 20728; DSMZ, Braunschweig, Germany) was included as an anaerobe representative.

Techniques: Transformation Assay, Light Microscopy

Journal: Journal of Clinical Microbiology

Article Title: Real-Time Optical Antimicrobial Susceptibility Testing

doi: 10.1128/JCM.00440-13

Figure Lengend Snippet: Bacterial growth of aerobic and anaerobic bacteria with (green line) or without (blue line) an antibiotic measured by the oCelloScope using the SESA algorithm. (a) S. aureus grown for 6 h with or without penicillin G; (b) E. coli grown for 6 h with or without polymyxin B; (c) S. Typhimurium grown for 6 h with or without spectinomycin; (d) S. alactolyticus grown for 6 h with or without rifampin. All experiments were done in triplicate, each consisting of eight iterations. Standard derivations are shown as error bars on the curves.

Article Snippet: The common Gram-positive pig gut bacterium Streptococcus alactolyticus (type strain DSM 20728; DSMZ, Braunschweig, Germany) was included as an anaerobe representative.

Techniques: Bacteria